General characteristics

IGHIGKIGL
# sequences644624111155103688
# productive sequences61296310769799617
# sequences with stop codon2964424033395
# out-of-frame sequences871014152016

Heatmap of VJ hit concentrations

SHM characteristics

V segmentJ segment
# SHMs14297442952934
# substitutions14092125952182
# deletions101647518
# insertions103670234
# synonymous SHMs3834588161259
# stop codon SHMs327292797

Heatmap of amino acid substitutions:

Heatmap of nucleotide substitutions:

Distribution of SHM in IGHV:

Distribution of SHM in IGKV:

Distribution of SHM in IGLV:

Distribution of insertion/deletion V SHM positions in read:

Distribution of lengths of insertion/deletion V SHMs:

CDR characteristics

IGHIGKIGL
# CDR1s2022572079876
max CDR1 abundance36240980613424
# CDR2s4389110971488
max CDR2 abundance251723751619090
# CDR3s398381184415797
max CDR3 abundance116872617808

IGH CDR1 length distribution:

Most IGH CDR1s (98.08%) have length 24 nt

Distribution of nucleotide abundance per position for IGH CDR1s of length 24 nt:

IGH CDR1s of length 24 nt are in-frame. Plot of the most abundant amino acids per position:

IGH CDR2 length distribution:

Most IGH CDR2s (92.62%) have length 24 nt

Distribution of nucleotide abundance per position for IGH CDR2s of length 24 nt:

IGH CDR2s of length 24 nt are in-frame. Plot of the most abundant amino acids per position:

IGH CDR3 length distribution:

Most IGH CDR3s (12.46%) have length 42 nt

Distribution of nucleotide abundance per position for IGH CDR3s of length 42 nt:

IGH CDR3s of length 42 nt are in-frame. Plot of the most abundant amino acids per position:

IGK CDR1 length distribution:

Most IGK CDR1s (65.72%) have length 18 nt

Distribution of nucleotide abundance per position for IGK CDR1s of length 18 nt:

IGK CDR1s of length 18 nt are in-frame. Plot of the most abundant amino acids per position:

IGK CDR2 length distribution:

Most IGK CDR2s (99.96%) have length 9 nt

Distribution of nucleotide abundance per position for IGK CDR2s of length 9 nt:

IGK CDR2s of length 9 nt are in-frame. Plot of the most abundant amino acids per position:

IGK CDR3 length distribution:

Most IGK CDR3s (61.8%) have length 27 nt

Distribution of nucleotide abundance per position for IGK CDR3s of length 27 nt:

IGK CDR3s of length 27 nt are in-frame. Plot of the most abundant amino acids per position:

IGL CDR1 length distribution:

Most IGL CDR1s (77.21%) have length 27 nt

Distribution of nucleotide abundance per position for IGL CDR1s of length 27 nt:

IGL CDR1s of length 27 nt are in-frame. Plot of the most abundant amino acids per position:

IGL CDR2 length distribution:

Most IGL CDR2s (98.37%) have length 9 nt

Distribution of nucleotide abundance per position for IGL CDR2s of length 9 nt:

IGL CDR2s of length 9 nt are in-frame. Plot of the most abundant amino acids per position:

IGL CDR3 length distribution:

Most IGL CDR3s (45.13%) have length 30 nt

Distribution of nucleotide abundance per position for IGL CDR3s of length 30 nt:

IGL CDR3s of length 30 nt are in-frame. Plot of the most abundant amino acids per position: